Multiple resistant Amaranthus powellii from Canada, Ontario

INTERNATIONAL HERBICIDE-RESISTANT WEED DATABASE

MULTIPLE RESISTANT POWELL AMARANTH
(Amaranthus powellii)

Multiple Resistance: 2 Sites of Action
Inhibition of Acetolactate Synthase HRAC Group 2 (Legacy B)
PSII inhibitors - Serine 264 Binders HRAC Group 5 (Legacy C1 C2)

Canada, Ontario

INTRODUCTION		POWELL AMARANTH
Powell Amaranth (Amaranthus powellii) is a dicot weed in the Amaranthaceae family. In Ontario this weed first evolved multiple resistance (to 2 herbicide sites of action) in 1998 and infests Corn (maize), Soybean, and Wheat. Multiple resistance has evolved to herbicides in the Groups 2 (Legacy B), and PSII inhibitors - Serine 264 Binders HRAC Group 5 (Legacy C1 C2). These particular biotypes are known to have resistance to atrazine, imazethapyr, and metribuzin and they may be cross-resistant to other herbicides in the Groups 2 (Legacy B), and PSII inhibitors - Serine 264 Binders HRAC Group 5 (Legacy C1 C2). The 'Group' letters/numbers that you see throughout this web site refer to the classification of herbicides by their site of action. To see a full list of herbicides and HRAC herbicide classifications click here.

QUIK STATS (last updated Dec 28, 2000 )
Common Name	Powell Amaranth
Species	Amaranthus powellii
Group	Inhibition of Acetolactate Synthase HRAC Group 2 (Legacy B) PSII inhibitors - Serine 264 Binders HRAC Group 5 (Legacy C1 C2)
Herbicides	atrazine, imazethapyr, and metribuzin
Location	Canada, Ontario
Year	1998
Situation(s)	Corn (maize), Soybean, and Wheat
Contributors - (Alphabetically)	Francois Tardif
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NOTES ABOUT THIS BIOTYPE

FIELD HISTORIES

Francois Tardif

We believe this case of multiple resistance is the result of sequential selection. The grower most likely had triazine resistance in his fields in the 1980s. Reliance on ALS inhibitors (mostly imazethapyr) increased in the 1990s and it is very likely that this biotype was reselected for imazethapyr resistance.

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GENERAL

Hugh Beckie

Ferguson, G. M., R. S. Diebold, F. J. Tardif, and A. S. Hamill. 2000. Populations of green pigweed (Amaranthus powellii) and redroot pigweed (Amaranthus retroflexus) in Ontario show different patterns of resistance to ALS-inhibiting herbicides and to triazines. Weed Sci. Soc. Am. Abstr. 40:47-48.

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MECHANISM

Francois Tardif

We know that this biotype has a mutation in the psbA gene that confers triazine resistance. It also has a mutation in the ALS gene (carboxy end mutation) that causes imidazolinone resistance.

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ONGOING RESEARCH

Francois Tardif

We are working on the fitness of this biotype.

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STUDENT CONTACTS

Francois Tardif

Three grad students contributed on this biotype as part of their M. Sc. theses. Gabrielle Ferguson did the initial work on this biotype, confirming ALS resistance. Kris McNaughton was able to sequence the ALS gene and confirm the mutation. SHane Diebold preformed the screen for ttriazine resistance and confirmed the presence of the nuclear gene mutation.

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ACADEMIC ASPECTS

Confirmation Tests

Greenhouse, and Laboratory trials comparing a known susceptible Powell Amaranth biotype with this Powell Amaranth biotype have been used to confirm resistance. For further information on the tests conducted please contact the local weed scientists that provided this information.

Genetics

Genetic studies on HRAC Group 2, 5 resistant Powell Amaranth indicate that the inheritance is determined by a two gene trait. There may be a note below or an article discussing the genetics of this biotype in the Fact Sheets and Other Literature

Mechanism of Resistance

Studies on the mechanism of resistance of multiple resistant Powell Amaranth from Ontario indicate that resistance is due to an altered target site. There may be a note below or an article discussing the mechanism of resistance in the Fact Sheets and Other Literature

Relative Fitness

There is no record of differences in fitness or competitiveness of these resistant biotypes when compared to that of normal susceptible biotypes. If you have any information pertaining to the fitness of multiple resistant Powell Amaranth from Ontario please update the database.

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CONTRIBUTING WEED SCIENTISTS

FRANCOIS TARDIF

Associate Professor University Of Guelph Department Of Plant Agriculture 2055 Radatz Ave Guelph, N1G 2W1, Ontario Canada Email Francois Tardif Web : Web Site Link

ACKNOWLEDGEMENTS
The Herbicide Resistance Action Committee, The Weed Science Society of America, and weed scientists in Ontario have been instrumental in providing you this information. Particular thanks is given to Francois Tardif for providing detailed information.

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Herbicide Resistant Powell Amaranth Globally
(Amaranthus powellii)

Country

FirstYear

Situation

Active Ingredients

Site of Action

Canada (Ontario)

1977

Corn (maize)

atrazine, and metribuzin

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

Canada (Ontario)

1998

Corn (maize), Cropland, and Soybean

imazethapyr, and thifensulfuron-methyl

Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

Canada (Ontario)

1998

Corn (maize), Soybean, and Wheat

atrazine, imazethapyr, and metribuzin

Multiple Resistance: 2 Sites of Action
Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)
PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

Canada (Ontario)

1999

Carrots

atrazine, linuron, monolinuron, and prometryne

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

Canada (Manitoba)

2008

Beans, and Wheat

thifensulfuron-methyl, and tribenuron-methyl

Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

Canada (Quebec)

2012

Carrots

linuron

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

Canada (Quebec)

2012

Soybean

imazethapyr

Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

Canada (Ontario)

2019

Soybean

aminocyclopyrachlor, dichlorprop, imazethapyr, MCPA, and mecoprop

Multiple Resistance: 2 Sites of Action
Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)
Auxin Mimics ( HRAC Group 4 (Legacy O)

Czech Republic

1989

Orchards

atrazine, cyanazine, prometryne, simazine, and terbutryne

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

France

1982

Corn (maize)

atrazine

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

Switzerland

1986

Corn (maize)

atrazine

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

United States (Washington)

1992

Mint

terbacil

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

United States (Ohio)

1996

Soybean

imazaquin

Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

United States (Michigan)

2001

Asparagus, and Nurseries

atrazine, and diuron

PSII inhibitors - Serine 264 Binders ( HRAC Group 5 (Legacy C1 C2)

Literature about Similar Cases

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Gaines, T. A. ; Ward, S. M. ; Bukun, B. ; Preston, C. ; Leach, J. E. ; Westra, P.. 2012. Interspecific hybridization transfers a previously unknown glyphosate resistance mechanism in Amaranthus species. Evolutionary Applications 5 : 29 - 38.

A previously unknown glyphosate resistance mechanism, amplification of the 5-enolpyruvyl shikimate-3-phosphate synthase gene, was recently reported in Amaranthus palmeri. This evolved mechanism could introgress to other weedy Amaranthus species through interspecific hybridization, representing an avenue for acquisition of a novel adaptive trait. The objective of this study was to evaluate the potential for this glyphosate resistance trait to transfer via pollen from A. palmeri to five other weedy Amaranthus species (Amaranthus hybridus, Amaranthus powellii, Amaranthus retroflexus, Amaranthus spinosus, and Amaranthus tuberculatus). Field and greenhouse crosses were conducted using glyphosate-resistant male A. palmeri as pollen donors and the other Amaranthus species as pollen recipients. Hybridization between A. palmeri and A. spinosus occurred with frequencies in the field studies ranging from <0.01% to 0.4%, and 1.4% in greenhouse crosses. A majority of the A. spinosus × A. palmeri hybrids grown to flowering were monoecious and produced viable seed. Hybridization occurred in the field study between A. palmeri and A. tuberculatus (<0.2%), and between A. palmeri and A. hybridus (<0.01%). This is the first documentation of hybridization between A. palmeri and both A. spinosus and A. hybridus..

Ashigh, J. ; Corbett, C. A. L. ; Smith, P. J. ; Laplante, J. ; Tardif, F. J.. 2009. Characterization and diagnostic tests of resistance to acetohydroxyacid synthase inhibitors due to an Asp₃₇₆Glu substitution in Amaranthus powellii. Pesticide Biochemistry and Physiology 95 : 38 - 46.

A population of Amaranthus powellii that had survived imazethapyr did not have any of the documented AHAS mutations causing resistance in this species in Ontario. The goal of this research was to confirm resistance in this population, determine its molecular basis and develop diagnostic tests. Whole plant dose-response experiments showed the resistant population had greater than 25-fold resistance to the selective agent imazethapyr at the whole plant level. Whole plant resistance to other herbicides ranged between 9-fold to flumetsulam and 85-fold to flucarbazone. This was confirmed at the enzyme level with resistance ranging between 25-fold to imazethapyr and 485-fold to thifensulfuron. AHAS from the resistant population had 16% higher specific activity than that of the susceptible population and it was also less sensitive to feedback inhibition by valine, leucine and isoleucine. Genomic DNA was extracted to PCR amplify and sequence the AHAS gene and to determine the applicability of diagnostic tests. Sequencing of the AHAS gene revealed a single point mutation of thymine₁₁₂₅ to guanine coding for an Asp₃₇₆Glu substitution. Two different diagnostic tests, restriction fragment length polymorphism and PCR amplification of specific allele, were able to correctly identify the resistant population from the susceptible control and from other resistant populations. Although this mutation appears to confer higher resistance to pyrithiobac and flucarbazone, two herbicides not registered in Eastern Canada, it endows plants with enough protection to allow survival to imazethapyr, the main selective agent in Ontario..

Ashigh, J., C. L. Corbett, P. J. Smith, J. Laplante, And F. J. Tardif.. 2009. Characterization and diagnostic tests of resistance to acetohydroxyacid synthase inhibitors due to an Asp376Glu substitution in Amaranthus powellii. Pesticide Biochemistry and Physiology 95 : 38 - 46.

A population of Amaranthus powellii that had survived imazethapyr did not have any of the documented AHAS mutations causing resistance in this species in Ontario. The goal of this research was to confirm resistance in this population, determine its molecular basis and develop diagnostic tests. Whole plant dose–response experiments showed the resistant population had greater than 25-fold resistance to the selective agent imazethapyr at the whole plant level. Whole plant resistance to other herbicides ranged between 9-fold to flumetsulam and 85-fold to flucarbazone. This was confirmed at the enzyme level with resistance ranging between 25-fold to imazethapyr and 485-fold to thifensulfuron. AHAS from the resistant population had 16% higher specific activity than that of the susceptible population and it was also less sensitive to feedback inhibition by valine, leucine and isoleucine. Genomic DNA was extracted to PCR amplify and sequence the AHAS gene and to determine the applicability of diagnostic tests. Sequencing of the AHAS gene revealed a single point mutation of thymine1125 to guanine coding for an Asp376Glu substitution. Two different diagnostic tests, restriction fragment length polymorphism and PCR amplification of specific allele, were able to correctly identify the resistant population from the susceptible control and from other resistant populations. Although this mutation appears to confer higher resistance to pyrithiobac and flucarbazone, two herbicides not registered in Eastern Canada, it endows plants with enough protection to allow survival to imazethapyr, the main selective agent in Ontario..

Corbett, C. A. L. ; Tardif, F. J.. 2008. Detection of resistance to acetohydroxyacid synthase inhibitors in Amaranthus sp. using DNA polymorphisms. Pesticide Biochemistry and Physiology 92 : 48 - 55.

Resistance to acetohydroxyacid synthase inhibitors is very widespread worldwide and is generally due to various point mutations in the gene coding for the target enzyme. Rapid resistance confirmation is key for the proper management of resistance. We aimed at determining whether two DNA-based tests, PCR-RFLP and PCR amplification of specific alleles (PASA), could reliably identify common AHAS mutations in Amaranthus sp., and whether individual tests could be multiplexed. DNA was extracted from eight populations of Amaranthus powellii and Amaranthus retroflexus possessing four different resistance alleles. Gains or losses of endonuclease recognition sites due to the presence of resistance alleles allowed reliable detection of all alleles. PASA used an allele-specific primer with a 3′-terminal mismatch to wildtype DNA to selectively amplify a resistance allele. All four resistance alleles were efficiently identified with PASA. Both tests were multiplexed to allow more efficient identification of resistance and up to four distinct mutations could be revealed by a single test. Those tests were relatively easy to use, inexpensive and allowed quick identification of resistance..

Tardif, F. J. ; Rajcan, I. ; Costea, M.. 2006. A mutation in the herbicide target site acetohydroxyacid synthase produces morphological and structural alterations and reduces fitness in Amaranthus powellii. New Phytologist 169 : 251 - 264.

We investigated the effect of a herbicide resistance-conferring mutation on fitness in Amaranthus powellii. Morphological and histological observations were made. Growth and leaf appearance were recorded for six resistant and six susceptible populations. The competitiveness of a susceptible population was compared with that of a resistant population using a replacement series experiment. Leaves of the resistant plants were distorted and much smaller than those of susceptible plants. Additionally, they exhibited an abnormal morphological and structural pattern consisting of a mosaic of heterogeneous areas in the same leaf blade. The roots and stems had similar structures in susceptible and resistant plants, but the former were up to four times more developed. The resistant plants were slower to develop and produced 67% less biomass and 58% lower leaf area than susceptible plants. Under competitive conditions, one susceptible population outperformed one resistant population by 7-15 times. The Trp₅₇₄Leu acetohydroxyacid synthase (AHAS) mutation appears to have considerable pleiotropic effects on the early growth and development of the plants which, in competitive conditions, greatly reduce fitness..

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