Multiple resistant Sorghum halepense from Argentina

INTERNATIONAL HERBICIDE-RESISTANT WEED DATABASE

MULTIPLE RESISTANT JOHNSONGRASS
(Sorghum halepense)

Multiple Resistance: 2 Sites of Action
Inhibition of Acetyl CoA Carboxylase HRAC Group 1 (Legacy A)
Inhibition of Enolpyruvyl Shikimate Phosphate Synthase HRAC Group 9 (Legacy G)

Argentina, Cordoba

INTRODUCTION		JOHNSONGRASS
Johnsongrass (Sorghum halepense) is a monocot weed in the Poaceae family. In Argentina this weed first evolved multiple resistance (to 2 herbicide sites of action) in 2015 and infests Soybean. Multiple resistance has evolved to herbicides in the Groups 1 (Legacy A), and Inhibition of Enolpyruvyl Shikimate Phosphate Synthase HRAC Group 9 (Legacy G). These particular biotypes are known to have resistance to clethodim, glyphosate, and haloxyfop-methyl and they may be cross-resistant to other herbicides in the Groups 1 (Legacy A), and Inhibition of Enolpyruvyl Shikimate Phosphate Synthase HRAC Group 9 (Legacy G). The 'Group' letters/numbers that you see throughout this web site refer to the classification of herbicides by their site of action. To see a full list of herbicides and HRAC herbicide classifications click here.

QUIK STATS (last updated Oct 25, 2021 )
Common Name	Johnsongrass
Species	Sorghum halepense
Group	Inhibition of Acetyl CoA Carboxylase HRAC Group 1 (Legacy A) Inhibition of Enolpyruvyl Shikimate Phosphate Synthase HRAC Group 9 (Legacy G)
Herbicides	clethodim, glyphosate, and haloxyfop-methyl
Location	Argentina, Cordoba
Year	2015
Situation(s)	Soybean
Contributors - (Alphabetically)	Julio Alejandro Scursoni, and Diego Ustarroz
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NOTES ABOUT THIS BIOTYPE

CROSS RESISTANCE

Julio Alejandro Scursoni

In 2019 a Sorghum halepense population from a soybean field in the province of Cordoba, was found to be resistant to glyphosate, haloxifop, and clethodim.

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GENERAL

Diego Ustarroz

This population is resistant to glyphosate and haloxyfop-metyl, however remains susceptible to clethodim.

See Spanish Paper Here

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ACADEMIC ASPECTS

Confirmation Tests

Greenhouse, and Laboratory trials comparing a known susceptible Johnsongrass biotype with this Johnsongrass biotype have been used to confirm resistance. For further information on the tests conducted please contact the local weed scientists that provided this information.

Genetics

Genetic studies on HRAC Group 1, 9 resistant Johnsongrass have not been reported to the site. There may be a note below or an article discussing the genetics of this biotype in the Fact Sheets and Other Literature

Mechanism of Resistance

The mechanism of resistance for this biotype is either unknown or has not been entered in the database. If you know anything about the mechanism of resistance for this biotype then please update the database.

Relative Fitness

There is no record of differences in fitness or competitiveness of these resistant biotypes when compared to that of normal susceptible biotypes. If you have any information pertaining to the fitness of multiple resistant Johnsongrass from Argentina please update the database.

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CONTRIBUTING WEED SCIENTISTS

JULIO ALEJANDRO SCURSONI

Professor University of Buenos Aires Dept. of Agronomy Av. San Martín 4453 Buenos Aires, C1417, Cordoba Argentina Email Julio Alejandro Scursoni

DIEGO USTARROZ

Research Associate Inta Sección Malezas Ruta 9 Km 636 Manfredi Córdoba Argentina. Manfredi, 5688, Córdoba Argentina Email Diego Ustarroz

ACKNOWLEDGEMENTS
The Herbicide Resistance Action Committee, The Weed Science Society of America, and weed scientists in Argentina have been instrumental in providing you this information. Particular thanks is given to Julio Alejandro Scursoni, and Diego Ustarroz for providing detailed information.

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Herbicide Resistant Johnsongrass Globally
(Sorghum halepense)

Country

FirstYear

Situation

Active Ingredients

Site of Action

Argentina

2005

Soybean

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

Argentina

2015

Soybean

haloxyfop-methyl

Inhibition of Acetyl CoA Carboxylase ( HRAC Group 1 (Legacy A)

Argentina

2015

Soybean

clethodim, glyphosate, and haloxyfop-methyl

Multiple Resistance: 2 Sites of Action
Inhibition of Acetyl CoA Carboxylase ( HRAC Group 1 (Legacy A)
Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

Australia (Queensland)

2019

Fallow

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

Chile

2009

Corn (maize)

nicosulfuron

Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

Greece

2005

Cotton

propaquizafop, and quizalofop-ethyl

Inhibition of Acetyl CoA Carboxylase ( HRAC Group 1 (Legacy A)

Hungary

2015

Corn (maize), and Fallow

foramsulfuron, and nicosulfuron

Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

Israel

2006

Carrots, Cotton, Onions, and Peanut (groundnut)

clethodim, fluazifop-butyl, and haloxyfop-methyl

Inhibition of Acetyl CoA Carboxylase ( HRAC Group 1 (Legacy A)

Israel

2017

Cotton, and Watermelon

rimsulfuron

Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

Italy

2005

Soybean, and Tomatoes

fluazifop-butyl, haloxyfop-methyl, propaquizafop, and quizalofop-ethyl

Inhibition of Acetyl CoA Carboxylase ( HRAC Group 1 (Legacy A)

Italy

2007

Corn (maize)

nicosulfuron

Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

Mexico

2009

Corn (maize)

foramsulfuron, nicosulfuron, primisulfuron-methyl, and rimsulfuron

Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

Serbia

2014

Corn (maize)

imazamox, nicosulfuron, propoxycarbazone-Na, pyroxsulam, and rimsulfuron

Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

Serbia

2017

Soybean

fenoxaprop-ethyl, fluazifop-butyl, haloxyfop-methyl, propaquizafop, and quizalofop-ethyl

Inhibition of Acetyl CoA Carboxylase ( HRAC Group 1 (Legacy A)

Serbia

2018

Soybean

fluazifop-butyl, and nicosulfuron

Multiple Resistance: 2 Sites of Action
Inhibition of Acetyl CoA Carboxylase ( HRAC Group 1 (Legacy A)
Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

Spain

2015

Corn (maize)

nicosulfuron

Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

United States (Mississippi)

1991

Cotton, and Cropland

fenoxaprop-ethyl, fluazifop-butyl, and quizalofop-ethyl

Inhibition of Acetyl CoA Carboxylase ( HRAC Group 1 (Legacy A)

United States (Kentucky)

1991

Soybean

fluazifop-butyl

Inhibition of Acetyl CoA Carboxylase ( HRAC Group 1 (Legacy A)

United States (Mississippi)

1992

Cotton

pendimethalin

Inhibition of Microtubule Assembly ( HRAC Group 3 (Legacy K1)

United States (Tennessee)

1995

Cotton, and Soybean

fluazifop-butyl, and quizalofop-ethyl

Inhibition of Acetyl CoA Carboxylase ( HRAC Group 1 (Legacy A)

United States (Virginia)

1995

Soybean

sethoxydim

Inhibition of Acetyl CoA Carboxylase ( HRAC Group 1 (Legacy A)

United States (Louisiana)

1997

Cotton

clethodim, and fluazifop-butyl

Inhibition of Acetyl CoA Carboxylase ( HRAC Group 1 (Legacy A)

United States (Texas)

2000

Corn (maize)

imazethapyr, and nicosulfuron

Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

United States (West Virginia)

2004

Corn (maize)

nicosulfuron

Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

United States (Indiana)

2005

Corn (maize), and Soybean

nicosulfuron

Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

United States (Kentucky)

2006

Corn (maize)

foramsulfuron, nicosulfuron, and primisulfuron-methyl

Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

United States (Arkansas)

2007

Soybean

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (Mississippi)

2008

Soybean

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (Louisiana)

2010

Soybean

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

United States (Missouri)

2019

Soybean

glyphosate

Inhibition of Enolpyruvyl Shikimate Phosphate Synthase ( HRAC Group 9 (Legacy G)

Venezuela

2010

Corn (maize)

foramsulfuron, iodosulfuron-methyl-Na, and nicosulfuron

Inhibition of Acetolactate Synthase ( HRAC Group 2 (Legacy B)

Literature about Similar Cases

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Hernandez, M.J., R. Leon, A. J. Fischer, M. Gebauer, R. Galdames, and R. FIgueroa. 2015. Target-Site Resistance to Nicosulfuron in Johnsongrass (Sorghum halepense) from Chilean Corn Fields.. Weed Science 63 : 631 - 640.

Johnsongrass is a common weed of corn in Chile, which is most often controlled by nicosulfuron, an acetohydroxyacid synthase (AHAS)-inhibiting herbicide. Recurrent nicosulfuron use has resulted in selection for resistant johnsongrass biotypes. We conducted studies to determine nicosulfuron resistance levels in two johnsongrass biotypes from Chile and to investigate if this resistance was target-site mediated. Whole-plant resistance to nicosulfuron was 33 and 46 times higher in resistant (R) than in susceptible (S) plants grown from seed and rhizomes, respectively. The nicosulfuron concentrations for 50% inhibition of AHAS enzyme activity in vitro were more than 11 times higher in R than in S plants. Sequencing analysis of the AHAS coding sequence revealed a Trp-574-Leu substitution in both R biotypes. This study shows that resistance to nicosulfuron in the two R biotypes is conferred by an altered target site. We also report the first consensus sequence of the johnsongrass AHAS gene corresponding to the known mutation sites conferring resistance to AHAS-inhibiting herbicides..

Ferraro, D. O. ; Ghersa, C. M.. 2013. Fuzzy assessment of herbicide resistance risk: glyphosate-resistant johnsongrass, Sorghum halepense (L.) Pers., in Argentina's croplands. Crop Protection 51 : 32 - 39.

A fuzzy-logic based model was built in order to assess the relative influence of different ecological and management drivers on glyphosate resistance risk (GRR) in Sorghum halepense (L.) Pers. The model was hierarchically structured in a bottom-up manner by combining 16 input variables throughout a logical network. Input data were related to (1) herbicide usage, (2) crop rotation, (3) landscape characterization, (4) weed dispersal, and (5) mean maximum and minimum seasonal temperature. Mean maximum and minimum seasonal temperatures and the dominance of glyphosate use were the variables that showed the highest sensitivity to input changes. Application of the model at a regional scale resulted in a wide range of GRR values. The lowest range values (lower than 0 and between 0 and 0.25) were represented in 5.5% and 21.5% of the cropping area, respectively. Intermediate GRR range (between 0.25 and 0.5) were assessed in 57.3% of the cropping area whilst the highest GRR range values (0.5-0.7) were represented in only 15.6% of the studied area. The assessment of trade-offs between different ecosystem functions through expert opinion can complement traditional analyses for predicting herbicide resistance risk based on solely the genetic aspect of the evolutionary process..

Vila-Aiub, M. M. ; Gundel, P. E. ; Yu Qin ; Powles, S. B.. 2013. Glyphosate resistance in Sorghum halepense and Lolium rigidum is reduced at suboptimal growing temperatures. Pest Management Science 69 : 228 - 232.

Background: Glyphosate resistance in populations of the C₄ perennial Sorghum halepense (Johnsongrass) and C₃ annual Lolium rigidum (rigid ryegrass) has evolved and been documented in many cropping areas around the globe. In S. halepense and in the majority of reported cases in L. rigidum the glyphosate resistance trait has been associated with a mechanism that reduces glyphosate translocation within plants. Here, the significant decrease in the glyphosate resistance level when resistant plants of S. halepense and L. rigidum are grown at suboptimal cool temperature conditions is reported. Results: Lowering temperature from 30 to 19°C in S. halepense and from 19 to 8°C in L. rigidum significantly reduced both plant survival and above-ground biomass produced by glyphosate-resistant plants. Thus, glyphosate resistance parameters significantly decreased when glyphosate-treated resistant plants of both species were grown under non-optimal temperature conditions. The results suggest that the resistance mechanism against glyphosate damage is less efficient at optimal growing temperatures. Conclusion: It is possible to increase the control of glyphosate-resistant S. halepense and L. rigidum populations by treatment with glyphosate during growing conditions at suboptimal low temperatures. Conversely, glyphosate failure will continue to occur on glyphosate-resistant populations treated during periods of higher temperatures..

Fernández, L. ; Haro, L. A. de ; Distefano, A. J. ; Martínez, M. C. ; Lía, V. ; Papa, J. C. ; Olea, I. ; Tosto, D. ; Hopp, H. E.. 2013. Population genetics structure of glyphosate-resistant Johnsongrass (Sorghum halepense L. Pers) does not support a single origin of the resistance. Ecology and Evolution 3 : 3388 - 3400.

Single sequence repeats (SSR) developed for Sorghum bicolor were used to characterize the genetic distance of 46 different Sorghum halepense (Johnsongrass) accessions from Argentina some of which have evolved toward glyphosate resistance. Since Johnsongrass is an allotetraploid and only one subgenome is homologous to cultivated sorghum, some SSR loci amplified up to two alleles while others (presumably more conserved loci) amplified up to four alleles. Twelve SSR providing information of 24 loci representative of Johnsongrass genome were selected for genetic distance characterization. All of them were highly polymorphic, which was evidenced by the number of different alleles found in the samples studied, in some of them up to 20. UPGMA and Mantel analysis showed that Johnsongrass glyphosate-resistant accessions that belong to different geographic regions do not share similar genetic backgrounds. In contrast, they show closer similarity to their neighboring susceptible counterparts. Discriminant Analysis of Principal Components using the clusters identified by K-means support the lack of a clear pattern of association among samples and resistance status or province of origin. Consequently, these results do not support a single genetic origin of glyphosate resistance. Nucleotide sequencing of the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) encoding gene from glyphosate-resistant and susceptible accessions collected from different geographic origins showed that none presented expected mutations in aminoacid positions 101 and 106 which are diagnostic of target-site resistance mechanism..

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