International Survey of Herbicide-Resistant Weeds

Countries Weeds Herbicides Mutations Graphs References Researchers
GROUP B/2 RESISTANT RED RICE
(Oryza sativa var. sylvatica)


ALS inhibitors (B/2)

United States, Arkansas
INTRODUCTION RED RICE
Red Rice (Oryza sativa var. sylvatica) is a monocot weed in the Poaceae family.  In United States this weed first evolved resistance to Group B/2 herbicides in 2002 and infests Rice.   Group B/2 herbicides are known as ALS inhibitors (Inhibition of acetolactate synthase ALS (acetohydroxyacid synthase AHAS)).  Research has shown that these particular biotypes are resistant to imazamox, imazapyr, imazaquin, imazethapyr, and pyrithiobac-sodium and they may be cross-resistant to other Group B/2 herbicides.

The 'Group' letters/numbers that you see throughout this web site refer to the classification of herbicides by their site of action. To see a full list of herbicides and HRAC herbicide classifications click here.

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QUIK STATS (last updated Aug 22, 2016 )

Common NameRed Rice
SpeciesOryza sativa var. sylvatica
GroupALS inhibitors (B/2)
Herbicidesimazamox, imazapyr, imazaquin, imazethapyr, and pyrithiobac-sodium
LocationUnited States
Year2002
Situation(s)Rice
Contributors - (Alphabetically)Nilda R. Burgos 
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NOTES ABOUT THIS BIOTYPE

GENETICS

Nilda R. Burgos

Weed Technology. 2006. Volume 20:576–584

Characterization of Spontaneous Crosses between Clearfield Rice (Oryza sativa) and Red Rice (Oryza sativa)

VINOD K. SHIVRAIN, NILDA R. BURGOS, KAREN A. K. MOLDENHAUER, RONALD W. MCNEW, and TOMILEA L. BALDWIN


Abstract: Experiments were conducted to determine the inheritance of resistance in crosses between imazethapyr-resistant rice and red rice. Past experiments on red rice control, using the Clearfield rice technology, resulted in outcrossing between Clearfield rice and Stuttgart strawhull red rice. The F2 generation of these spontaneous crosses were characterized with respect to inheritance of imazethapyr resistance, leaf color and leaf pubescence, and seed shattering, pubescence, color, and size. Agronomic traits of hybrids were also observed in relation to their parents. To determine the segregation of resistance among F2 phenotypes, the response of three- to four-leaf plants to imazethapyr was scored 3 wk after application as resistant (R, no imazethapyr symptoms), susceptible (S, death of plants), or intermediate (I, stunted plants). R, I, and S phenotypes segregated in a 1:2:1 ratio in the F2 generation. Two- or three-gene inheritance was documented for leaf and seed characteristics. A wide range in onset of flowering (70 to 130 d after planting) was observed in F2 families, although 6% of the plants did not flower during the growing season. F2 plants were taller and had more tillers than any of their parents. Resistance to imazethapyr is associated with a single, incompletely dominant allele. 

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MECHANISM

Nilda R. Burgos

Weed Science, 53:567–577. 2005

Mutations in the red rice ALS gene associated with resistance to imazethapyr


Satyendra N. Rajguru Crop, Soil, and Environmental Sciences, 1366 West Altheimer Drive, University of Arkansas, Fayetteville, AR 72704
Nilda R. Burgos Corresponding author. Crop, Soil, and Environmental Sciences, 1366 West Altheimer Drive, University of Arkansas, Fayetteville, AR 72704; nburgos@uark.edu
Vinod K. Shivrain Crop, Soil, and Environmental Sciences, 1366 West Altheimer Drive, University of Arkansas, Fayetteville, AR 72704
James McD. Stewart Crop, Soil, and Environmental Sciences, PTSC115, University of Arkansas, Fayetteville, AR 72701


The introduction of Clearfield (CL) rice cultivars resistant to imidazolinone herbicides, acetolactate synthase (ALS) inhibitors, has raised concerns of gene flow to weedy rice genotypes collectively called ‘‘red rice’’ that infest rice-growing areas in the southern United States. This experiment was conducted to study hybridization between CL rice and red rice using simple sequence repeats (SSR) markers, identify mutations in the ALS gene of imazethapyr-resistant red rice, and to detect the introgression of the ALS-resistant gene from CL rice into red rice. Natural outcrossing experiments between CL rice and strawhull (SH) red rice were set up in Stuttgart, AR, in 2002 and 2003. Putative red rice hybrids were detected among volunteer plants in the following year. Hybridization was confirmed using SSR markers, and introgression of the resistant ALS gene from CL rice to red rice was detected by ALS gene sequencing. The ALS gene sequences of U.S. rice cultivars ‘Bengal’ and ‘Cypress’, SH red rice, CL rice (CL161), and imazethapyr-resistant red rice/CL rice hybrids were compared. Nucleotide sequences of the ALS gene from the rice cultivars were identical. Three point mutations were present in the SH red rice ALS gene coding region relative to Bengal/Cypress. One of these resulted in the substitution of Asp630 for Glu630. The ALS gene sequences of confirmed hybrids were identical to that of the herbicide-resistant pollen source, CL161. We identified four ALS gene mutations in the herbicide-resistant red rice hybrids relative to the susceptible rice cultivars. One point mutation, resulting in a substitution of Ser653 with Asn, was linked to ALS resistance in callus tissue derived from a Kinmaze rice line from Japan. The other three mutations (Ser186—Pro, Lys416—Glu, and Leu662—Pro) are novel. This experiment confirmed that gene flow from imidazolinone-resistant rice resulted in herbicide-resistant red rice plants.

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MECHANISM

Nilda R. Burgos

Weed Science 2008 56:485–489

Amino Acid Substitutions in the Acetolactate Synthase Gene of Red Rice (Oryza sativa) Confer Resistance to Imazethapyr

 
Marites A. Sales, Vinod K. Shivrain, Nilda R. Burgos, and Yong I. Kuk


Two red rice accessions from Arkansas have been found to be resistant to the labeled rate of imazethapyr, which is used to control red rice in ClearfieldTM rice. Full-length amplification of the acetolactate synthase (ALS) gene in imazethapyrresistant red rice revealed a coding sequence of 1,935 base pairs, which is the same as that of the cultivated rice. Coding sequences were generated from four red rice accessions collected from different geographical regions in Arkansas, consisting of accessions that were either resistant or susceptible to imazethapyr. Nucleotide sequence alignments identified six base polymorphisms, three of which resulted in amino acid substitutions in the ALS gene. One amino acid substitution, Gly654Glu, involves a residue required for imazethapyr binding to the ALS. The other substitution, Val669Met, implies conformational changes in the ALS structure that enhances binding of thiamine diphosphate, an ALS cofactor. These novel amino acid substitutions first reported for ALS-resistant red rice accessions support the hypothesis that ALS-resistant red rice can evolve with sustained herbicide selection pressure. Thus, it behooves growers to integrate the Cleafield rice technology with other tools to achieve a successful, long-term weed management program.

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RESISTANCE RATIO

Nilda R. Burgos

Weed Science 2008 56:1–11

Natural Tolerance to Imazethapyr in Red Rice (Oryza sativa)

Yong I. Kuk, Nilda R. Burgos, and Vinod K. Shivrain


Red rice is a major weed problem in rice production of the southern United States and other rice-producing countries. One hundred thirty red rice accessions from 26 rice-growing counties in Arkansas were tested for tolerance to imazethapyr in seed- and whole-plant response bioassays. The red rice accessions were compared with imazethapyr-resistant (ClearfieldTM) rice cultivars (‘CL121’, ‘CL161’, and ‘CL-XL8’) and conventional rice cultivars (‘Bengal’, ‘Dongjin’, ‘Drew’, and ‘Wells’). Red rice accessions 79, 84, and 118 showed 17-, 48-, and 37-fold more tolerance to imazethapyr, respectively, than the standard susceptible red rice accession (82) in whole-plant bioassays. The imazethapyr-resistant rice cultivars, CL121, CL161, and CL-XL8 were 41-, .177-, and 48-fold more resistant to imazethapyr, respectively than the susceptible standard. The imazethapyr-tolerant red rice and ClearfieldTM cultivars were generally cross tolerant to other acetolactate synthase (ALS; EC 4.1.3.18) inhibiting herbicides such as imazapyr, imazaquin, imazamox, and pyrithiobac. The tolerance level of red rice or rice to imidazolinone herbicides was highest with imazaquin and lowest with imazapyr. The imazethapyr-tolerant red rice accessions and ClearfieldTM rice were susceptible to glufosinate and glyphosate. The ALS enzyme of tolerant red rice accessions was less sensitive to imazethapyr than the susceptible standard, but tolerance at the enzyme level was less than at the whole-plant level. Therefore, tolerance of red rice to imazethapyr may involve other mechanisms besides an insensitive target site. We learned that a few imazethapyr-tolerant red rice populations existed probably before ClearfieldTM rice was introduced, supporting the hypothesis that evolution of herbicide-resistant red rice populations can happen with intensive herbicide selection pressure. 

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ACADEMIC ASPECTS

Confirmation Tests

Field, and Greenhouse trials comparing a known susceptible Red Rice biotype with this Red Rice biotype have been used to confirm resistance. For further information on the tests conducted please contact the local weed scientists that provided this information.
 
Genetics

Genetic studies on Group B/2 resistant Red Rice have not been reported to the site.  There may be a note below or an article discussing the genetics of this biotype in the Fact Sheets and Other Literature
 
Mechanism of Resistance

Studies on the mechanism of resistance of Group B/2 resistant Red Rice from United States indicate that resistance is due to an altered target site.  There may be a note below or an article discussing the mechanism of resistance in the Fact Sheets and Other Literature
 
Relative Fitness

There is no record of differences in fitness or competitiveness of these resistant biotypes when compared to that of normal susceptible biotypes.  If you have any information pertaining to the fitness of Group B/2 resistant Red Rice from United States please update the database.
 
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CONTRIBUTING WEED SCIENTISTS

NILDA R. BURGOS
Professor - Weed Physiology
University Of Arkansas, Fayetteville
Crop, Soil
And Environmental Sciences
1366 W. Altheimer Dr.
Fayetteville, 72704, Arkansas
United States
Email Nilda R. Burgos
Web   : Web Site Link

ACKNOWLEDGEMENTS

The Herbicide Resistance Action Committee, The Weed Science Society of America, and weed scientists in United States have been instrumental in providing you this information. Particular thanks is given to Nilda R. Burgos for providing detailed information.
Herbicide Resistant Red Rice Globally
(Oryza sativa var. sylvatica)
Herbicide Resistant Red Rice Globally
(Oryza sativa var. sylvatica)
Drag a column header and drop it here to group by that column
Herbicide Resistant Red Rice Globally
(Oryza sativa var. sylvatica)
#CountryFirstYearSituationActive IngredientsSite of Action
1 BrazilBrazil 2006 Rice imazapic, and imazethapyr 5 ALS inhibitors (B/2)
242Oryza sativa var. sylvaticaRed Rice5372
2 Costa RicaCosta Rica 2010 Rice imazapic, and imazapyr 11 ALS inhibitors (B/2)
242Oryza sativa var. sylvaticaRed Rice7885
3 GreeceGreece 2013 Rice imazamox, and imazethapyr 19 ALS inhibitors (B/2)
242Oryza sativa var. sylvaticaRed Rice13054
4 ItalyItaly 2010 Rice imazamox 24 ALS inhibitors (B/2)
242Oryza sativa var. sylvaticaRed Rice5614
5 United States (Arkansas) United StatesArkansas2002 Rice imazamox, imazapyr, imazaquin, imazethapyr, and pyrithiobac-sodium 45 ALS inhibitors (B/2)
242Oryza sativa var. sylvaticaRed Rice13046
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