CATCHWEED BEDSTRAW (Galium aparine) with GROUP B/2 resistance: (INHIBITION OF ACETOLACTATE SYNTHASE ) Inhibition of Acetolactate Synthase
MUTATION: PROLINE 197 to THREONINE
Catchweed Bedstraw (Galium aparine) is a dicot plant in the rubiaceae family. A single amino acid substitution from Proline 197 to Threonine has led to resistance to Inhibition of Acetolactate Synthase as indicated in the table below.		Catchweed Bedstraw

Chemical Family	Example Herbicide	Resistance Level
Imidazolinones	Imazethapyr	Not Determined
Pyrimidinyl benzoates	Bispyribac-Na	Not Determined
Sulfonylureas	Chlorsulfuron	Resistant > 10 fold
Triazolopyrimidine - Type 1	Chloransulam-methyl	Not Determined
Triazolinones	Flucarbazone-Na	Not Determined

REFERENCES

Zhang, L. L., Q. Li, W. L. Guo, W. Li, and J. X. Wang. 2016. Molecular mechanism of Galium aparine resistance to tribenuron-methyl. Chinese Agricultural Science Bulletin 32 : 110 - 113.

In recent years,the tribenuron-methylin resistant Galium aparine was serious throughout HenanProvince,and common dose of tribenuron-methyl cannot control Galium aparine any more.The objective ofthis study is to understand the molecular mechanism of the Galium aparine resistance to tribenuron-methyl.Through total DNA extraction,ALS clone and sequencing,the authors found the specific mutation sites inamino acid sequence of acetolactate synthase(ALS) in the resistant biotype of Galium aparine.Compared withthe susceptible biotype,there were three types of amino acid substitutions in the resistant biotypes,of which,the amino acid substitution of Pro197(CCC) to Thr(ACC) and Pro197(CCC) to Leu(CTC) were located in thehighly conserved region Domain A,Thr 457(ACC) to Ser(TCC) in the non-conservative region.Thesubstitution of Pro197 might be the reason for the resistance in the R-biotype of Galium aparine to tribenuron-methyl..

This case was entered by Patrick Tranel Email: tranel@illinois.edu

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