GOOSEGRASS (Eleusine indica) with GROUP G/9 resistance: (INHIBITION OF ENOLPYRUVYL SHIKIMATE PHOSPHATE SYNTHASE ) Inhibition of EPSP synthase
MUTATION: PROLINE 106 to THREONINE
Goosegrass (Eleusine indica) is a monocot plant in the poaceae family. A single amino acid substitution from Proline 106 to Threonine has led to resistance to Inhibition of Enolpyruvyl Shikimate Phosphate Synthase as indicated in the table below.		Goosegrass

Chemical Family	Example Herbicide	Resistance Level
Glycines	glyphosate	Intermediate Resistance < 10 fold

REFERENCES

Ng, C. H. ; Wickneswari, R. ; Salmijah, S. ; Teng, Y. T. ; Ismail, B. S.. 2003. Gene polymorphisms in glyphosate-resistant and -susceptible biotypes of Eleusine indica from Malaysia. Weed Research (Oxford) 43 : 108 - 115.

Resistant (R) and susceptible (S) biotypes of Eleusine indica were collected from four areas, namely Chaah, Lenggeng, Bidor and Temerloh, in Malaysia. Restriction fragment length polymorphism (RFLP) and polymerase chain reaction (PCR)-RFLP analyses using SphI restriction enzyme were able to differentiate the R biotype from the S biotype by showing R-specific and S-specific polymorphisms in E. indica from three of the areas, with the exception of Temerloh where no polymorphisms were detected. The different DNA profiles for the R biotypes obtained indicate that SphI is not a useful diagnostic marker. The DNA polymorphisms detected in the 5-enolpyruvylshikimate 3-phosphate (EPSP) synthase gene suggest that there are different mutation events leading to development of resistance to glyphosate. Partial sequencing of the EPSP synthase gene confirmed different mutations occurring with substitution of proline with serine or threonine at amino acid 106 for the R biotype in Chaah, Bidor and Temerloh..

This case was entered by Todd Gaines Email: todd.gaines@colostate.edu

PERMISSION MUST BE OBTAINED FIRST if you intend to base a significant portion of a scientific paper on data derived from this site.
Cite this site as: Heap, I.  The International Survey of Herbicide Resistant Weeds.  Online.  Internet.  Saturday, November 23, 2024 .  Available  www.weedscience.org
Copyright © 1993- 2024 WeedScience.org All rights reserved. Fair use of this material is encouraged. Proper citation is requested.